Equipments-复旦大学智能医学研究院网站(英文)
Equipments

1. Bio-Rad RT-PCR

Bio-Rad CFX Duet RT-PCR is a model of advanced instruments for real-time polymerase chain reaction, which is designed for high-efficient, precise and flexible detection on nuclear acids. This instrument is equipped with unique dual detection channels, which are able to detect two different signals, largely promoting the efficiency of experiments. The high-level sensitivity and accuracy of this instrument facilitates the wide-ranged applications in molecular biology, clinical diagnosis, and biological medicine.

In addition, Bio-Rad CFX Duet RT-PCR is equipped with a straight user interface and robust data analytic capability. The users can easily set parameters and supervise the reactive processes. The equipped advanced optical system and accurate thermos-controlling technique produce reliable and repeatable results. Moreover, the detection on multiple fluorescent probes is also compatible with this instrument, which fulfill various experimental requirements.

Besides the capabilities listed above, this instrument is also highly automatized, which leads to simplified processes and saved time. Therefore, by the excellent performance and convenient operations, this instrument has provided a reliable solution for research personnels and clinic laboratory groups who have requirements for molecular biological analyses.

48E98

2.Tsingtao Haier Large High-Speed Multifunctional Centrifuge with refrigeration

This Tsingtao Haier Model LX-155T500R is a centrifuge of high performance, which can be widely applicable to biological medicine, biological laboratories and clinical diagnoses. With unique designs, this centrifuge is equipped with extra-large sample cells of 500 mL which can contain samples with large volumes. Installed with a brushless DC motor and micro-chips, this instrument can precisely control centrifugation at high speed. An intuitive and clean interface of the control panel on a LED screen provides convenience for users to easily set parameters like RPM/RCF values and duration time, which is largely personalized. Besides, this model instrument is equipped with multiple safety systems, including an over-speed protection and a door-locking function, which confirm the security and stability during the operation. By the outstanding performance, reliable safety and flexibility, the centrifuge of the model LX-155T500R has become an indispensable and important instrument in a research laboratory.  


3.Diagenode Bioruptor Pico ultra-sonicator

Diagenode Bioruptor Pico is a high-end and advanced sonicator for homogenizing samples, which has been widely applied to molecular biology and genetics. With a unique ultrasonic technique, homogenization can be efficiently performed by this instrument to release DNA, RNA and proteins from lysed cells, providing samples with high quality for the following experiments. Besides, this instrument is capable of concentrating the energy from ultrasonic waves to efficiently fragmentate gDNA into a relatively narrowed basepair range, which induces itself to become a powerful instrument for ChIP-Seq.

The outstanding control function of this instrument has provided an opportunity for users to precisely adjust watts of ultrasound, duration time and temperature in order to fulfill requirements for different parameters and promote the efficiency. The operation on this instrument has been simplified with an installed LED touch screen with a user-friendly software interface, providing an opportunity for users to supervise the process of ultrasonication.

4.Merck-Millipore Milli-Q IQ-7500 Ultrapure Water System

This usystem can produce ultrapure water of top-level quality for biological laboratory. Once pressing the button, water at required flow speed will be generated. And the flow speed can be adjusted by drops with a maximum speed at 2 liters per minute. Otherwise, the pure water can be also generated by directly operating the touch screen.

Fundamental Parameters:

1. Electric resistance in water: 18.2 MΩ when at 25℃

2. Total organic carbon (TOC): 1-5 ppb (input water: < 50 ppb). An internal TOC sensor is installed, including a 0.5 mL quartz sample cell, a 172nm UV lamp, titanium electrodes, electromagnetic valves and a temperature compensation unit. TOC detection range: 0.5-999 ppb, accuracy: ±0.1 ppb.

3. Granules: granules of diameter > 0.22 μm: < 1 /mL.

4. Microbes: < 0.01 CFU/mL. Under a specific condition: < 0.005 CFU/mL

5. Pyrogen (endotoxic): < 0.001 EU/mL

6. RNase: < 1 pg/mL

7. DNase: < 5 pg/mL

8. Protease: < 0.15 μg/mL

9. Water flow speed: drops by drops ~ 2 liters/min

5.Olympus IXplore 73 Inverted Florescent Microscope

This microscope is widely applied to life sciences, clinic medicine and drug development based on its imagination unit of high-level sensitivity and modularized designs. Some key features are included as the core advantages of this instruments:

Real-time live-cell imagination: This microscope is capable of observing adhesive living cells growing on the bottoms of plates and bottles by its inverted optical routine, which is also capable of long-time imagination when equipped with a small incubator, facilitating the research on cell migration and proliferation.

Multiple fluorescent imagination: This microscope is equipped with a LED lamp of high lumen and multiple filters, which provides an opportunity to detect the signals of up to 4 fluorescent markers. This ability fulfills the microscopy requirements for the co-localization imagination with dyes like GFP and DAPI.

6.ThermoFisher NanoDroop One Micro-Volume Spectrophotometer

This micro-volume spectrophotometer is the latest model of the NanoDrop series. This instrumental mechanism is related to liquid surface tension, and the loaded volumes for samples are usually required for 0.5 ~ 2.0 μL. On the loading board, by the fixed length of photo routine between up- and down-arms, sample detection becomes rapid, with low volumes, high concentrated, without quartz cells, and without capillaries. The design of this instrument is widely welcome in the world as the accuracy and conveniency of this machine provide high efficiency for scientists during their research work. The equipped pulsed xenon flash lamp has offered a wide wavelength range from 190 to 840 nm for detection. Moreover, the warm-up is unnecessary for the xenon lamp, which can be ready to serve immediately after power-on. Equipped with a high-sensitive CCD array detector, the maximum detectible absorbance value is as high as 300 Abs (dsDNA concentration: 2~15,000 ng/μL). Therefore, a direct loading, without dilution, is suitable for most nuclear acid samples after purification

Typical samples:

Nuclear acids: dsDNA, ssDNA, RNA (absorbance at 260 and 280 nm)

Probes: the absorbance of fluorescent-labelled probes

Cell density: the absorbance of the cell suspension, at 600 nm

Proteins: the absorbance of purified proteins at 280 nm

7.ThermoFisher NanoDroop One Micro-Volume Spectrophotometer

This ELISA microplate reader is a model of high performance, which is widely applied in the research of biological medicine, biological pharmaceutics and clinical diagnosis. This model works precisely, reliably and efficiently, fulfilling various ELISA requirements. This Bio-Rad model is equipped with a high-end optical system and an precise liquid transferring system, both of which provide accurate detection results. The detection unit is highly sensitive with a wide linear range, which is qualified for detecting samples of low concentrations. Moreover, the optical routine can perform auto-calibration and auto-adjustment, also endorsing the accuracy for detecting samples.

This instrument is composed of a main body, micro-chip control unit and software, providing a convenient and friendly software interface for users. Multiple experimental modes are compatible with this instrument, including ELISA, ELISPOT, et al..

Parameters:

Wavelength range: 400-750 nm

Absorbance detection range: 0.0 - 3.5 OD

Accuracy: ±1.0% or 0.010 OD when at 490nm with range 0.0003.000 OD

Filters: 8 filters, including 415, 450, 490, 595, 655 and 750 nm

Shaking mode: low, medium, high mode Duration time range: 0-999 sec.

Plate reading duration: solo wavelength: 6 sec dual wavelengths: 10 sec




















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