Wu Feizhen

Researcher; Smart Pharmacy, Bioinformatics

Unit

Fudan University, Intelligent Medicine Instistute

Educational experience

· 2006/09-2009/07, Shanghai University, Center for Bioelectronics, Electronic Biology, Ph.D.

· 2003/09-2006/03, Shanghai University, Center for Bioelectronics, Computational Software and Theory, Master.

· 1996/09-2002/07, Zhejiang University, School of Medicine, Graduated from Clinical Medicine, Bachelor.

Study Abroad Experience

· 2015/09-2017/02, Dana-Farber Institute of Harvard University, visiting scholar (national government).

· 2009/11-2010/01, visiting scholar at UC San Diego/NIH/Harvard University.

Work Experience

· 2022/11-present, Intelligent Medicine Instistute, researcher.

· 2021/01-2022/11, Fudan University Biomedical Research Institute, researcher.

· 2013/01-2020/12, Associate Researcher, Institute of Biomedical Sciences, Fudan University.

· 2009/09-2012/12, Assistant Researcher, Institute of Biomedical Sciences, Fudan University.

Research Profile

Mainly engaged in frontier basic scientific research of epigenetics and bioinformatics, and achieved a series of important research results. In 2011, he first reported the molecular mechanism of TET1 protein regulating DNA hydroxymethylation in mouse embryonic stem cells; in 2012, he clarified the characteristics of DNA methylation and hydroxymethylation changes in melanoma, and clarified the DNA hydroxymethylation signal The relationship between changes and melanoma; recently, it was reported that NONO protein interacted with TET1 to affect the differentiation of mouse embryonic stem cells into neural cells; DNA methylation and hydroxymethylation in neural cell models and brain tissues of Alzheimer's disease were revealed Genome distribution characteristics of Alzheimer's disease and its role in judging the occurrence of Alzheimer's disease; a new DNA methylation detection method was invented, which can detect DNA mutation and DNA methylation simultaneously in one experiment, and can be used For the accurate detection of genetic changes and epigenetic changes, the method has obtained patent authorization in China and the United States. In addition, the mechanism of action of CD151 and its complex signaling network in the occurrence and development of liver cancer was also studied. Published more than 40 high-level SCI papers, the Google Scholar Index (H-index) was 23, and the cumulative number of citations of the articles reached 4196. The research project has been funded by the National "863", the National Major Research Program, the National Natural Science Foundation of China, and the Shanghai Natural Science Foundation of China.

Paper Published

Published more than 40 SCI papers in high-level journals such as Cell, Nature, Science Advances, Nucleic Acids Research, Molecular Cell, and Hepatology. 10 papers signed by co-first author or co-corresponding author, 6 of which have SCI impact factor (IF)>10, and 15 other signed papers. At present, the Google Scholar Index (H-index) is 23, and the articles have been cited more than 4,000 times, including 2 papers with more than 600 citations and 13 papers with more than 100 citations.

Articles signed by the corresponding author or the first author

1. Aye L., Wang Z., Chen F., Xiong Y., Zhou J., Wu Feizhen#, Hu L. #, and Wang D. #, Circadian Regulator-Mediated Molecular Subtypes Depict the Features of Tumor Microenvironment and Indicate Prognosis in Head and Neck Squamous Cell Carcinoma. J Immunol Res, 2023. 2023: p. 9946911. (#共同通讯作者,IF 4.1)

2. Ning Li, Kangkang Yu, Minhui Dong, Jinyu Wang, Feifei Yang, Haoxiang Zhu, Jie Yu, Jingshu Yang, Wentao Xie, Bidisha Mitra, Richeng Mao, Feizhen Wu#, Haitao Guo#, Jiming Zhang#. Intrahepatic transcriptomics reveals gene signatures in chronic hepatitis B patients responded to interferon therapy. Emerging Microbes & Infections, 2022; 11(1) (#共同通讯作者,IF 19.568)

3. Xiaoqiang Chai, Jianfei Guo, Ruizhao Dong, Xuan Yang, Chao Deng, Chuanyuan Wei, JiaJie Xu, Weiyu Han, Jiacheng Lu, Chao Gao, Dongmei Gao, Cheng Huang, Aiwu Ke, Shuangqi Li, Huanping Li, Yingming Tian, Zhongkai Gu, Shuxian Liu, Hang Liu, Qilong Chen, Feng Liu, Jian Zhou, Jia Fan, Guoming Shi,Feizhen Wu#, Jiabin Cai#. Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B‐related hepatocellular carcinoma.Clinical and translational medicine, 2021; 11(3) (#共同通讯作者,IF7.8)

4. Dingailu Ma, Irfete S Fetahu, Mei Wang, Rui Fang, Jiahui Li, Hang Liu, Tobin Gramyk, Isabella Iwanicki, Sophie Gu, Winnie Xu, Li Tan, Feizhen Wu # , Yujiang G Shi # . The fusiform gyrus exhibits an epigenetic signature for Alzheimer's disease. Clinical Epigenetics , 27 August 2020 (#co-corresponding author, IF 5.028)

5. Shuangqi Li*, Jiuxing Feng*,Feizhen Wu*#, Jiabin Cai, Xinyu Zhang, Haikun Wang, Irfete S Fetahu, Isabella Iwanicki, Dingailu Ma, Tao Hu, Hang Liu, Bingjie Wang, Guoming Shi#, Li Tan#, Yujiang Geno Shi#.TET2 promotes anti‐tumor immunity by governing G‐MDSCs and CD8+ T‐cell numbers.EMBO reports, 2020 (#共同一作且共同通讯作者,IF7.497)

6. Wenjing Li * , Violetta Karwacki-Neisius * # , Chun Ma, Li Tan, Yang Shi, Feizhen Wu # and Yujiang Geno Shi # . Nono deficiency compromises TET1 chromatin association and impedes neuronal differentiation of mouse embryonic stem cells. , 2020 (#co-corresponding author, IF 11.501 )

7. Irfete S Fetahu, Dingailu Ma, Kimberlie Rabidou, Christian Argueta, Michael Smith, Hang Liu, Feizhen Wu # , Yujiang G Shi # . Epigenetic signatures of methylated DNA cytosine in Alzheimer's disease. Science Advances 2019; 5(8) ( #co-corresponding author, IF 13.116)

8. Jin Li, Yan Li, Wei Li, Huaibing Luo, Yanping Xi, Shihua Dong, Gao M, Xu P , Zhang B , Liang Y , Zou Q , Hu X , Peng L , Zou D , Wang T , Yang H , Jiang C , Peng SL # , Wu Feizhen # Yu WQ # . Guide Positioning Sequencing identifies aberrant DNA methylation patterns that alter cell identity and tumor-immune surveillance networks. Genome Research. 2019; 1(22) (#co-corresponding author, IF 11.093)

9. Wenqi Xu , Jiahui Li , Bowen Rong, Hongjie Shen, Bin Zhao, Mei Wang, Qilong Chen, Hang Liu, Zhongkai Gu, Shuxian Liu, Rui Guo, Feizhen Wu # , Fei Lan # . DNMT3A Reads and Connects Histone H3K36me2 to DNA methylation. Protein & Cell .2020 Feb;11(2):150-154 (#co-corresponding author, IF 10.164)

10.Peng L,Li Y,Xi Y,Li W,Li J, Lv R, Zhang L,Zou Q, Dong S,Luo H,Wu Feizhen#,Yu W#. Methyl-CpG-binding domain protein 3-like 2 (MBD3L2) promotes Tet2 enzymatic activity for mediating 5mC oxidation.J Cell Sci2016 Jan 14. (#共同通讯作者,IF 4.7)

11. Xu Y*,Wu Feizhen*, Tan L*, Kong L, Xiong L, Deng J, Barbera AJ, Zheng L, Zhang H, Huang S, Min J, Nicholson T, Chen T, Xu G, Shi Y, et al.Genome-wide regulation of 5hmC, 5mC, and gene expression by Tet1 hydroxylase in mouse embryonic stem cells.Molecullar Cell2011;42(4):451-464. (*共同第一作者, IF15.584)

12. Ke AW*, Shi GM*, Zhou J*,Wu Feizhen*, Ding ZB, Hu MY, Xu Y, Song ZJ, Wang ZJ, Wu JC, Bai DS, Li JC, Liu KD, Fan J. Role of overexpression of CD151 and/or c-Met in predicting prognosis of hepatocellular carcinoma.Hepatology2009;49(2):491-503.(*共同第一作者,IF 14.679)

13. Devbhandari RP*, Shi GM*, Ke AW*,Wu Feizhen*, Huang XY, Wang XY, Shi YH, Ding ZB, Xu Y, Dai Z, Fan J, Zhou J. Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin beta1 complex in the progression of hepatocellular carcinoma.PLoS One2011;6(9):e24901. (*共同第一作者,IF 4.092)

Articles signed by  other author 

14. Wenqi Xu, Jiahui Li, Chenxi He, Jing Wen, Honghui Ma, Bowen Rong, Jianbo Diao, Liyong Wang, Jiahua Wang,Feizhen Wu, Li Tan, Yujiang Geno Shi, Yang Shi, Hongjie Shen. METTL3 regulates heterochromatin in mouse embryonic stem cells.Nature2021.

15. Yangliu Song, Weicheng Chen, Zitong Huang, Guixiang Tian, Mengru Li, Zhengshan Zhao, Zhiyu Feng, Feizhen Wu, Maoxiang Qian, Xiaojing Ma, Wei Sheng, Guoying Huang. A Non-coding HES1 Variant Predisposes Children to Congenital Heart Disease in Chinese Population.Frontiers in cell and developmental biology. 2021.

16. Wu, D., Hu, D., Chen, H., Shi, G., Fetahu, I. S.,Wu Feizhen, Rabidou, K., Fang, R., …, Lan, F., Fan, J., Shi, Y., Shi, Y. G. Glucose-regulated phosphorylation of TET2 by AMPK reveals a pathway linking diabetes to cancer.Nature. 2018 Jul (IF 41.57)

17. Ying Wang, Chao Yin, Zhidan Chen, Yang Li, Yan Zou, Xiang Wang, Yanpeng An,Feizhen Wu, Guoping Zhang, Chunjie Yang, Huiru Tang, Yunzeng Zou, Hui Gong. Cardiac-specific LRP6 knockout induces lipid accumulation through Drp1/CPT1b pathway in adult mice.Cell and tissue research.2019

18. Lian CG, Xu Y, Ceol C,Wu Feizhen, Larson A, Dresser K, Xu W, Tan L, Hu Y, Zhan Q, Lee CW, Hu D, Lian BQ, Kleffel S, Yang Y, et al.Loss of 5-hydroxymethylcytosine is an epigenetic hallmark of melanoma.Cell2012;150(6):1135-1146. (IF 31.957)

19. Xu Y, Xu C, Kato A, Tempel W, Abreu JG, Bian C, Hu Y, Hu D, Zhao B, Cerovina T, Diao J,Wu Feizhen, He HH, Cui Q, Clark E, et al.Tet3 CXXC domain and dioxygenase activity cooperatively regulate key genes for Xenopus eye and neural development.Cell2012;151(6):1200-1213. (IF 31.957)

20. Dango S, Mosammaparast N, Sowa ME, Xiong LJ,Wu Feizhen, Park K, Rubin M, Gygi S, Harper JW, Shi Y. DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation.Mol Cell2011;44(3):373-384. (IF 14.178)

21. Chen T, Huang Z, Wang L, Wang Y,Wu Feizhen, Meng S, Wang C. MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages.Cardiovasc Res. 2009;83(1):131-139. (IF 5.8)

22. Rajakumara E, Wang Z, Ma H, Hu L, Chen H, Lin Y, Guo R,Wu Feizhen, Li H, Lan F, Shi YG, Xu Y, Patel DJ, Shi Y. PHD finger recognition of unmodified histone H3R2 links UHRF1 to regulation of euchromatic gene expression.Mol Cell2011;43(2):275-284. (IF 14.178)

23. Chen S, Ma J,Wu Feizhen, Xiong LJ, Ma H, Xu W, Lv R, Li X, Villen J, Gygi SP, Liu XS, Shi Y. The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcriptional elongation.Genes Dev2012;26(12):1364-1375. (IF 12.64)

24. Tan L, Xiong L, Xu W,Wu Feizhen, Huang N, Xu Y, Kong L, Zheng L, Schwartz L, Shi Y, Shi YG. Genome-wide comparison of DNA hydroxymethylation in mouse embryonic stem cells and neural progenitor cells by a new comparative hMeDIP-seq method.Nucleic Acids Res2013;41(7):e84. (IF 10.16)

25. Q. Gao, Y. J. Zhao, X. Y. Wang, W. J. Guo, S. Gao, L. Wei, J. Y. Shi, G. M. Shi, Z. C. Wang, Y. N. Zhang, Y. H. Shi, J. Ding, Z. B. Ding, A. W. Ke, Z. Dai,Wu Feizhen, H. Wang, Z. P. Qiu, Z. A. Chen, Z. F. Zhang, S. J. Qiu, J. Zhou, X. H. He and J. Fan.Activating mutations in PTPN3 promote cholangiocarcinoma cell proliferation and migration and are associated with tumor recurrence in patients.Gastroenterology2014;146(5):1397-1407. (IF 18.39)

26. Guo R, Zheng L, Park JW, Lv R, Chen H, Jiao F, Xu W, Mu S, Wen H, Qiu J, Wang Z, Yang P,Wu Feizhen, Hui J, Fu X, et al.BS69/ZMYND11 reads and connects histone H3.3 lysine 36 trimethylation-decorated chromatin to regulated pre-mRNA processing.Mol Cell2014;56(2):298-310. (IF 14.178)

27. Laurent B, Ruitu L, Murn J, Hempel K, Ferrao R, Xiang Y, Liu S, Garcia BA, Wu H,Wu Feizhen, Steen H, Shi Y. A Specific LSD1/KDM1A Isoform Regulates Neuronal Differentiation through H3K9 Demethylation.Mol Cell2015;57(6):957-970 (IF 14.178)

28. Chun Ma, Violetta Karwacki-Neisius, Haoran Tang, Wenjing Li, Zhennan Shi, Haolin Hu, Wenqi Xu, Zhentian Wang, Lingchun Kong, Ruitu Lv, Zheng Fan, Wenhao Zhou, Pengyuan Yang,Wu Feizhen, Jianbo Diao, Li Tan, Yujiang Geno Shi, Fei Lan, Yang Shi.Nono, a bivalent domain factor, regulates Erk signaling and mouse embryonic stem cell pluripotency.Cell reports2016;17(4): 997-1007 (IF8.28)

29. Xiao M, Li J, Li W, Wang Y,Wu Feizhen, Xi Y, Zhang L, Ding C, Luo H, Li Y, Peng L, Zhao L, Peng S, Xiao Y, Dong S, Cao J, Yu W. MicroRNAs activate gene transcription epigenetically as an enhancer trigger.RNA Biol.2016 Feb 6:1-9. (IF3.9)

30.Shen H,Xu W,Guo R,Rong B,Gu L,Wang Z,He C,Zheng L,Hu X,Hu Z,Shao ZM,Yang P,Wu Feizhen,Shi YG,Shi Y,Lan F. Suppression of Enhancer Overactivation by a RACK7 Histone Demethylase Complex.Cell.2016 Apr 7;165(2):331-42. (IF 31.957)

31. Kong L, Tan L, Lv R, Shi Z, Xiong L,Wu Feizhen, Rabidou K, Smith M, He C, Zhang L, Qian Y, Ma D, Lan F, Shi Y, Shi YG. A primary role of TET proteins in establishment and maintenance of De Novo bivalency at CpG islands.Nucleic Acids Res.2016 Oct 14;44(18):8682-8692. (IF 10.16)